Fusobacterium nucleatum Promotes Exosomal LncRNA MANCR Secretion from Colorectal Cancer Cells to Induce PD-L1 Expression in Macrophages
Menée à l'aide de modèles murins et à partir de l'analyse de 54 échantillons cliniques de cancer colorectal, cette étude met en évidence un mécanisme par lequel la bactérie Fusobacterium nucleatum induit l'expression du ligand PD-L1 sur les macrophages en favorisant la sécrétion, par les cellules cancéreuses via des exosomes, du long ARN non codant associé à la mitose
Fusobacterium nucleatum is a key microbial driver of colorectal cancer progression. In this study, we identified an intercellular signaling axis through which F. nucleatum remodels the immune microenvironment. Analysis of 54 clinical colorectal cancer specimens revealed that high intratumoral F. nucleatum load correlated significantly with PD-L1 upregulation in tumor-associated macrophages, diminished CD8+ T-cell cytotoxicity, and poor patient prognosis. Mechanistically, F. nucleatum infection activated NF-
κB signaling in colorectal cancer cells to induce the transcription of the long noncoding RNA mitotically associated long noncoding RNA (MANCR), which was selectively packaged into exosomes via the ESCRT-III/ALIX complex and transferred to macrophages. In recipient macrophages, exosomal MANCR interacted with HNRNP U to increase PD-L1 mRNA stability, leading to sustained PD-L1 surface expression. In humanized mouse models, F. nucleatum exposure inhibited CD8+ T-cell infiltration and suppressed granzyme B activity, thereby compromising antitumor immunity and facilitating tumor proliferation and metastasis. These findings demonstrate that F. nucleatum exploits a tumor-derived exosomal long noncoding RNA network to remotely manipulate macrophage plasticity. Therefore, targeting the F. nucleatum/MANCR/PD-L1 axis may represent a viable strategy to overcome immune resistance in the colorectal cancer microenvironment.
Cancer Research , résumé, 2026