Development and Validation of a Computational Histology Artificial Intelligence–Powered Predictive Biomarker for Selection of Chemotherapy in Advanced Pancreatic Cancer
Menée à partir d'une approche computationnelle et de lames histologiques d'échantillons biopsiques colorés à l'éosine-hématoxyline et issus de 477 patients atteints d'un cancer du pancréas de stade avancé traité par chimiothérapie de première ligne (fluoropyrimidine ou gemcitabine), cette étude évalue la performance d'un système de score basé sur des caractéristiques histomorphologiques pour prédire la survie globale ou le délai avant second traitement ou décès
Purpose : Predictive biomarkers to guide selection of first-line chemotherapy for advanced pancreatic ductal adenocarcinoma (PDAC) are an unmet clinical need. This study used the Computational Histology Artificial Intelligence (CHAI) platform to develop and validate a histomorphology-based G-chemo versus F-chemo (GvF) biomarker that predicts benefit from first-line fluoropyrimidine-based (F-chemo) versus gemcitabine-based (G-chemo) regimens.
Methods : The CHAI platform extracted quantitative histomorphologic features from whole-slide images of hematoxylin and eosin–stained diagnostic biopsies. In a multi-institutional development cohort, features associated with differential outcomes as measured by time to next treatment or death (TNTD) between F-chemo–treated and G-chemo–treated patients produced continuous biomarker scores, which were dichotomized into G-pref or F-pref results. The biomarker and threshold were locked. An independent validation cohort from the prospective COMPASS and Know Your Tumor studies assessed differential treatment outcomes by TNTD and overall survival (OS).
Results : There were 477 patients (development: 178; validation: 299). In validation, among 173 F-pref patients, those treated with F-chemo had significantly better outcomes than G-chemo for both TNTD (P = .035; median TNTD: F-chemo 8.6 months; G-chemo 7.5 months) and OS (P = .003; median OS: F-chemo 14.4 months; G-chemo 11.7 months). Among 126 G-pref patients, G-chemo had significantly superior TNTD (P = .038; median TNTD: F-chemo 7.2 months; G-chemo 9.6 months), but no difference in OS (P = .5; median OS: F-chemo 12.4 months; G-chemo 14.3 months). In propensity score–weighted analysis, the biomarker predicted treatment effect (biomarker-treatment interaction TNTD P < .001; OS P = .005). RNA subtypes were associated with TNTD and OS but did not predict differential treatment effects (P = .3).
Conclusion : The histomorphology-based GvF biomarker predicted differential treatment benefit of first-line GvF. This biomarker can guide optimal treatment selection for first-line therapy in advanced PDAC.
Journal of Clinical Oncology , article en libre accès, 2026