Single-cell analysis: toward the clinic
Cet article passe en revue les perspectives offertes par les plateformes d'analyse protéomique à l'échelle d'une seule cellule pour l'étude des réseaux de signalisation dans le cancer
Human life starts with a single cell, the zygote, which undergoes multiple mitotic cell divisions to form a complete body. During each cell division, the genome has to be replicated to form the approximately 1014 cells that make up the adult human body. For a long time, it was thought that all cells from the same individual harbored identical genomes. The only known exceptions being lymphocytes, in which antigen- and pathogen-driven recombination and hypermutation generate genetic diversity, and germline cells, which undergo meiotic recombination.
During normal mitotic cell division, however, the genome is not replicated with absolute precision; this results in the incorporation of somatic mutations...
Genome Medicine , éditorial en libre accès, 2012