Invited Perspective: Prioritizing Chemical Testing and Evaluation Using Validated in Vitro Assays Relevant to Key Characteristics
Menée aux Etats-Unis à partir des données d'un essai in vitro de stéroïdogenèse à haut débit, cette étude identifie 296 produits chimiques qui augmentent la synthèse d'oestradiol et/ou de progestérone puis analyse les sources d'exposition potentielles à ces produits
By interfering with hormone action, endocrine-disrupting chemicals (EDCs) can increase the risk of various adverse health outcomes, including cancer and reproductive impairment (La Merrill et al. 2020). In their article, Cardona and Rudel (2021) have identified nearly 300 chemicals that increased estradiol, progesterone, or both in an in vitro steroidogenesis assay that is internationally validated for use in regulatory contexts. They screened publicly available testing data for more than 2,000 chemicals tested in the ToxCast™ high-throughput in vitro steroidogenesis assay in cultured human H295R adrenocarcinoma cells. This U.S. Environmental Protection Agency Tier 1 assay has been used to study chemical impacts on 13 hormones involved in the steroidogenic pathway, including estrogens and progestogens (Haggard et al. 2018; Karmaus et al. 2016). Cardona and Rudel focused specifically on estradiol and progesterone. For the active agents, they systematically compiled available in vivo evidence from databases, authoritative evaluations, and published studies. An important consideration was whether in vivo effects were reported in the rodent mammary gland. This well-studied model system has supported classifications of reproductive toxicants and carcinogens (Rudel et al. 2011). The authors also leveraged information on exposure to provide context for their findings.
Environmental Health Perspectives , éditorial en libre accès, 2020