Characterisation of circulating tumour cell phenotypes identifies a partial-EMT sub-population for clinical stratification of pancreatic cancer
Menée à partir de 272 échantillons sanguins prélevés sur 74 patients atteints d'un adénocarcinome canalaire du pancréas, cette étude identifie une sous-population de cellules tumorales circulantes présentant une transition épithélio-mésenchymateuse partielle et met en évidence une corrélation entre cette sous-population et le pronostic
Background : Limited accessibility of the tumour precludes longitudinal characterisation for therapy guidance in pancreatic ductal adenocarcinoma (PDAC).
Methods : We utilised dielectrophoresis-field flow fractionation (DEP-FFF) to isolate circulating tumour cells (CTCs) in 272 blood draws from 74 PDAC patients (41 localised, 33 metastatic) to non-invasively monitor disease progression.
Results : Analysis using multiplex imaging flow cytometry revealed four distinct sub-populations of CTCs: epithelial (E-CTC), mesenchymal (M-CTC), partial epithelial-mesenchymal transition (pEMT-CTC) and stem cell-like (SC-CTC). Overall, CTC detection rate was 76.8% (209/272 draws) and total CTC counts did not correlate with any clinicopathological variables. However, the proportion of pEMT-CTCs (prop-pEMT) was correlated with advanced disease, worse progression-free and overall survival in all patients, and earlier recurrence after resection.
Conclusion : Our results underscore the importance of immunophenotyping and quantifying specific CTC sub-populations in PDAC.
British Journal of Cancer , résumé, 2021