miR-622 is a novel potential biomarker of breast carcinoma and impairs motility of breast cancer cells through targeting NUAK1 kinase
Menée à l'aide d'échantillons plasmatiques prélevés sur 17 témoins et 39 patientes atteintes d'un carcinome canalaire invasif du sein et menée à partir d'échantillons tumoraux prélevés sur 20 patientes supplémentaires, cette étude met en évidence une association entre le niveau du microARN miR-622 et les caractéristiques de la tumeur (stade, niveau de l'antigène nucléaire Ki67) puis démontre que le microARN miR-622, en réduisant l'expression de la kinase NUAK1, affecte la motilité des cellules cancéreuses
Background : Aberrant expression of microRNAs (miR) has been proposed as non-invasive biomarkers for breast cancers. The aim of this study was to analyse the miR-622 level in the plasma and in tissues of breast cancer patients and to explore the role of miR-622 and its target, the NUAK1 kinase, in this context.
Methods : miR-622 expression was analysed in plasma and in tissues samples of breast cancer patients by q-RT-PCR. Bioinformatics programs, luciferase assay, public dataset analysis and functional experiments were used to uncover the role of miR-622 and its target in breast cancer cells.
Results : miR-622 is downregulated in plasma and in tissues of breast cancer patients respect to healthy controls and its downregulation is significantly associated with advanced grade and high Ki67 level. Modulation of miR-622 affects the motility phenotype of breast cancer cells. NUAK1 kinase is a functional target of miR-622, it is associated with poor clinical outcomes of breast cancer patients and is inversely correlated with miR-622 level.
Conclusions : miR-622/NUAK1 axis is deregulated in breast cancer patients and affects the motility phenotype of breast cancer cells. Importantly, miR-622 and NUAK1 hold promises as biomarkers and as targets for breast cancers.
British Journal of Cancer , Article en libre accès, 2020