Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses
A partir d'échantillons de tissu tumoral, de tissu sain et de cellules sanguines prélevés sur 28 patients atteints d'un adénocarcinome du poumon, cette étude cartographie la diversité des cellules immunitaires présentes dans le micro-environnement tumoral
To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell analysis of the tumor, non-involved lung, and blood cells, we provide a detailed immune cell atlas of early lung tumors. We show that stage I lung adenocarcinoma lesions already harbor significantly altered T cell and NK cell compartments. Moreover, we identified changes in tumor-infiltrating myeloid cell (TIM) subsets that likely compromise anti-tumor T cell immunity. Paired single-cell analyses thus offer valuable knowledge of tumor-driven immune changes, providing a powerful tool for the rational design of immune therapies.
Cell , article en libre accès, 2016