Validation of biomarkers that complement CA19.9 in detecting early pancreatic cancer
Menée à partir d'échantillons sériques prélevés sur 250 patients atteints d'un adénocarcinome canalaire du pancréas, 130 patients présentant une maladie bénigne du pancréas et 20 personnes saines, cette étude met en évidence l'intérêt des biomarqueurs CA125 et LAMC2, en complément de CA19.9, pour détecter précocement la maladie
Purpose : Pancreatic ductal adenocarcinoma (PDAC) is a significant cause of cancer mortality. CA19.9, the only tumor marker available to detect and monitor PDAC, is not sufficiently sensitive and specific to consistently differentiate early cancer from benign disease. In this study we aimed to validate recently discovered serum protein biomarkers for the early detection of PDAC and ultimately develop a biomarker panel that could discriminate PDAC from other benign disease better than the existing marker CA19.9.
Experimental Design : We performed a retrospective blinded evaluation of 400 serum samples collected from individuals recruited on a consecutive basis. The sample population consisted of 250 individuals with PDAC at various stages, 130 individuals with benign conditions and 20 healthy individuals. The serum levels of each biomarker were determined by ELISAs or automated immunoassay.
Results : By randomly splitting matched samples into a training (n=186) and validation (n=214) set we were able to develop and validate a biomarker panel consisting of CA19.9, CA125 and LAMC2 that significantly improved the performance of CA19.9 alone. Improved discrimination was observed in the validation set between all PDAC and benign conditions (AUCCA19.9=0.80 versus AUCCA19.9+CA125+LAMC2= 0.87; p<0.005) as well as between early-stage PDAC and benign conditions (AUCCA19.9 = 0.69 versus AUCCA19.9+CA125+LAMC2 = 0.76; p<0.05) and between early-stage PDAC and chronic pancreatitis (AUCCA19.9 = 0.59 versus AUCCA19.9+CA125+LAMC2 = 0.74; p<0.05).
Conclusions : The data demonstrate that a serum protein biomarker panel consisting of CA125, CA19.9 and LAMC2 is able to significantly improve upon the performance of CA19.9 alone in detecting PDAC.
Clinical Cancer Research , résumé, 2014