Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells
Menée à l'aide de xénogreffes de gliomes, cette étude évalue les performances d'une technique d'imagerie, impliquant tomographie par émission de positrons et tomographie moléculaire de fluorescence dans le proche infra-rouge, pour détecter de façon non invasive les cellules souches cancéreuses surexprimant AC133
A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection of AC133+ tumor stem cells by PET and near-infrared fluorescence molecular tomography in subcutaneous and orthotopic glioma xenografts using antibody-based tracers. Particularly, microPET with 64Cu-NOTA-AC133 mAb yielded high-quality images with outstanding tumor-to-background contrast, clearly delineating subcutaneous tumor stem cell-derived xenografts from surrounding tissues. Intracerebral tumors as small as 2–3 mm also were clearly discernible, and the microPET images reflected the invasive growth pattern of orthotopic cancer stem cell-derived tumors with low density of AC133+ cells. These data provide a basis for further preclinical and clinical use of the developed tracers for high-sensitivity and high-resolution monitoring of AC133+ tumor stem cells.
Proceedings of the National Academy of Sciences , article en libre accès, 2014