RNA-guided diagnostics and therapeutics for next-generation individualized nanomedicine
Menée sur des échantillons tumoraux inclus en paraffine après prélèvement sur des patients atteints d'un carcinome basocellulaire (4) ou d'un carcinome à cellules de Merkel (12), cette étude évalue les performances d'une méthode à base d'hybridation in situ en fluorescence multicolore de micro-ARNs pour le diagnostic différentiel des deux types de cancer cutané
The absence of reliable quantitative laboratory tests for measurements of microRNAs and other classes of small noncoding RNAs in archived, formalin-fixed, paraffin-embedded human samples with sufficient specificity and sensitivity has significantly limited the development of clinically relevant noncoding RNA–based diagnostic and therapeutic applications. A report by Renwick et al. in this issue of the JCI presents a significant technical and methodological advance toward the development of reliable clinical laboratory-compatible multicolor RNA FISH methodology for molecular diagnostic applications and the near-term prospect of introduction of microRNA-based biomarkers into clinical practice. Further, this work is likely to advance the development of RNA-based therapeutics and next-generation individualized nanomedicine.
The Journal of Clinical Investigation , commentaire, 2012