2-hydroxyglutarate detection by magnetic resonance spectroscopy in IDH-mutated patients with gliomas
Menée sur 30 patients atteints d'un gliome, cette étude évalue la faisabilité d'une technique non invasive, la spectroscopie par résonance magnétique du proton, pour la détection d'une enzyme associée à la présence de mutations des gènes IDH1 et IDH2
Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been shown to be present in most World Health Organization grade 2 and grade 3 gliomas in adults. These mutations are associated with the accumulation of 2-hydroxyglutarate (2HG) in the tumor. Here we report the noninvasive detection of 2HG by proton magnetic resonance spectroscopy (MRS). We developed and optimized the pulse sequence with numerical and phantom analyses for 2HG detection, and we estimated the concentrations of 2HG using spectral fitting in the tumors of 30 subjects. Detection of 2HG correlated with mutations in IDH1 or IDH2 and with increased levels of D-2HG by mass spectrometry of the resected tumors. Noninvasive detection of 2HG may prove to be a valuable diagnostic and prognostic biomarker.
Nature Medicine , résumé, 2011