Circulation of Progenitor Cells in Obese and Lean Colorectal Cancer Patients
Cette étude (45 cas) évalue l'intérêt de mesurer le taux sanguin de cellules progénitrices et endothéliales CD34+ chez les patients maigres ou obèses pour le diagnostic d'un cancer colorectal
Background: Colorectal cancer (CRC) is a common life-threatening malignancy; risk and progression are elevated in obesity. The purpose of this study was to measure the frequency of circulating CD34 positive endothelial and progenitor cells in the circulation, and evaluate their potential values as CRC biomarkers. Methods: Blood was collected from 45 CRC patients and compared to cancer-free control donors. Detection and enumeration of cells was performed by using flow cytometry based on immunophenotypes established for the cell populations of interest: hematopoietic and endothelial circulating progenitor cells (CPC), endothelial cells (EC), mesenchymal stromal cells (MSC) and CD34bright leukocytes (CD34b LC). Groups were compared using multivariate regression analysis. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic values. Results: After adjusting for age and BMI, the mean frequencies of MSC and CD34bLC were significantly higher in the circulation of CRC patients compared to controls. The areas under the ROC curve were 0.77 and 0.82 for MSC, and CD34b LC, respectively. The frequency of circulating MSC, but not of the other cell populations, was also found to be significantly higher in the circulation of obese CRC patients (BMI ≥ 30 kg/m2) compared to lean CRC patients and obese controls. Conclusions: Increased frequency of MSC and CD34b LC in the peripheral blood may represent a new diagnostic marker for CRC. Impact: BMI-dependent changes in circulating MSC, potentially mobilized from white adipose tissue may reveal their trafficking to tumors, which could be one of the mechanistic links between obesity and cancer progression.
Cancer Epidemiology Biomarkers & Prevention , résumé, 2011